fatp1 inhibitor Search Results


fatp1 inhibitor  (MedChemExpress)
91
MedChemExpress fatp1 inhibitor
CD8 + T-cell function is impaired by BM long-chain fatty acid uptake via <t>FATP1.</t> (A-D) BM mononuclear cells from controls (n = 6) or patients with MM who had relapsed (n = 6) were cultured in autologous PB or BM plasma as indicated for 72 hours with stimulation via CD3/CD28. (A) CD8 + T-cell mitochondrial mass was assessed by flow cytometry using MVG and is shown for each group (left) and both groups combined (right). (B) At 72 hours, T cells were activated with PMA/ionomycin and expression of IFN-γ was assessed within CD8 + T cells by flow cytometry. (C-D) IFN-γ and TNF-α in cell culture supernatants was measured by ELISA. (E-H) BM mononuclear cells were cultured and assessed as in panels A-D but in either complete or lipid-depleted BM autologous plasma as indicated. (I-K) BM mononuclear cells from patients with MM at diagnosis (n = 6) were cultured in autologous PB (I,K) or BM (J-K) plasma as in panels A-D, in absence or presence of ferrostatin and assessed for IFN-γ expression by ELISA. (L) Proportion of CD8 + T cells expressing CD36 within indicated BM mononuclear cell samples from controls (n = 10), individuals with MGUS (n = 8), asymptomatic MM (asymptomatic; n = 9), or MM at diagnosis (diagnosis; n = 7) were assessed by flow cytometry. (M) Expression of IFN-γ, TNF-α, and IL-2 by CD36 + or CD36 − CD8 + T cells within indicated BM mononuclear cell samples as in panel L. (N-O) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous BM plasma as in panel A-D, in absence or presence of the CD36 inhibitor, SSO, and assessed for IFN-γ expression within CD8 + T cells by flow cytometry (N) or by ELISA (O). (P) single-cell RNA-sequencing UMAP projections of BM mononuclear cells from 2 patients with newly diagnosed MM, with cell type annotation (top left) and transcript abundance of indicated genes overlaid. (Q-S) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous PB (Q,S) or BM (R-S) plasma as in panels A-D, in absence or presence of the FATP1 inhibitor, FATP1in2, and assessed for IFN-γ expression by ELISA. Significance was calculated using Wilcoxon matched-pairs signed rank test for panels A-H (circles), I-J,N-O,Q-R; and paired t test for panels K,S; ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .0001. UMAP, Uniform Manifold Approximation and Projection.
Fatp1 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fatp1+inhibitor/FATP1-IN-2/pmc10582277-36-43-47
Average 91 stars, based on 1 article reviews
fatp1 inhibitor - by Bioz Stars, 2026-09
91/100 stars
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fatp1-in-2  (MuseChem Chemicals)
N/A
FATP1-IN-2（Cat No.:I045246）is a selective small-molecule inhibitor of fatty acid transport protein 1 (FATP1, also known as SLC27A1), a membrane protein that mediates the uptake and activation of long-chain fatty acids. By blocking FATP1 activity, FATP1-IN-2
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gene exp fabp5 hs02339439 g1  (Thermo Fisher)
88
Thermo Fisher gene exp fabp5 hs02339439 g1
CD8 + T-cell function is impaired by BM long-chain fatty acid uptake via <t>FATP1.</t> (A-D) BM mononuclear cells from controls (n = 6) or patients with MM who had relapsed (n = 6) were cultured in autologous PB or BM plasma as indicated for 72 hours with stimulation via CD3/CD28. (A) CD8 + T-cell mitochondrial mass was assessed by flow cytometry using MVG and is shown for each group (left) and both groups combined (right). (B) At 72 hours, T cells were activated with PMA/ionomycin and expression of IFN-γ was assessed within CD8 + T cells by flow cytometry. (C-D) IFN-γ and TNF-α in cell culture supernatants was measured by ELISA. (E-H) BM mononuclear cells were cultured and assessed as in panels A-D but in either complete or lipid-depleted BM autologous plasma as indicated. (I-K) BM mononuclear cells from patients with MM at diagnosis (n = 6) were cultured in autologous PB (I,K) or BM (J-K) plasma as in panels A-D, in absence or presence of ferrostatin and assessed for IFN-γ expression by ELISA. (L) Proportion of CD8 + T cells expressing CD36 within indicated BM mononuclear cell samples from controls (n = 10), individuals with MGUS (n = 8), asymptomatic MM (asymptomatic; n = 9), or MM at diagnosis (diagnosis; n = 7) were assessed by flow cytometry. (M) Expression of IFN-γ, TNF-α, and IL-2 by CD36 + or CD36 − CD8 + T cells within indicated BM mononuclear cell samples as in panel L. (N-O) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous BM plasma as in panel A-D, in absence or presence of the CD36 inhibitor, SSO, and assessed for IFN-γ expression within CD8 + T cells by flow cytometry (N) or by ELISA (O). (P) single-cell RNA-sequencing UMAP projections of BM mononuclear cells from 2 patients with newly diagnosed MM, with cell type annotation (top left) and transcript abundance of indicated genes overlaid. (Q-S) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous PB (Q,S) or BM (R-S) plasma as in panels A-D, in absence or presence of the FATP1 inhibitor, FATP1in2, and assessed for IFN-γ expression by ELISA. Significance was calculated using Wilcoxon matched-pairs signed rank test for panels A-H (circles), I-J,N-O,Q-R; and paired t test for panels K,S; ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .0001. UMAP, Uniform Manifold Approximation and Projection.
Gene Exp Fabp5 Hs02339439 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fatp1+inhibitor/Gene+Exp%2E+FABP5%2C+Hs02339439_g1/pm25413343-71-76--1
Average 88 stars, based on 1 article reviews
gene exp fabp5 hs02339439 g1 - by Bioz Stars, 2026-09
88/100 stars
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fatp1-in-1  (MuseChem Chemicals)
N/A
FATP1-IN-1（Cat No.:I045247）is a potent and selective inhibitor of fatty acid transport protein 1 (FATP1/SLC27A1), a key mediator of long-chain fatty acid uptake and activation in cells. By blocking FATP1 function, it reduces fatty acid transport
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Image Search Results


CD8 + T-cell function is impaired by BM long-chain fatty acid uptake via FATP1. (A-D) BM mononuclear cells from controls (n = 6) or patients with MM who had relapsed (n = 6) were cultured in autologous PB or BM plasma as indicated for 72 hours with stimulation via CD3/CD28. (A) CD8 + T-cell mitochondrial mass was assessed by flow cytometry using MVG and is shown for each group (left) and both groups combined (right). (B) At 72 hours, T cells were activated with PMA/ionomycin and expression of IFN-γ was assessed within CD8 + T cells by flow cytometry. (C-D) IFN-γ and TNF-α in cell culture supernatants was measured by ELISA. (E-H) BM mononuclear cells were cultured and assessed as in panels A-D but in either complete or lipid-depleted BM autologous plasma as indicated. (I-K) BM mononuclear cells from patients with MM at diagnosis (n = 6) were cultured in autologous PB (I,K) or BM (J-K) plasma as in panels A-D, in absence or presence of ferrostatin and assessed for IFN-γ expression by ELISA. (L) Proportion of CD8 + T cells expressing CD36 within indicated BM mononuclear cell samples from controls (n = 10), individuals with MGUS (n = 8), asymptomatic MM (asymptomatic; n = 9), or MM at diagnosis (diagnosis; n = 7) were assessed by flow cytometry. (M) Expression of IFN-γ, TNF-α, and IL-2 by CD36 + or CD36 − CD8 + T cells within indicated BM mononuclear cell samples as in panel L. (N-O) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous BM plasma as in panel A-D, in absence or presence of the CD36 inhibitor, SSO, and assessed for IFN-γ expression within CD8 + T cells by flow cytometry (N) or by ELISA (O). (P) single-cell RNA-sequencing UMAP projections of BM mononuclear cells from 2 patients with newly diagnosed MM, with cell type annotation (top left) and transcript abundance of indicated genes overlaid. (Q-S) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous PB (Q,S) or BM (R-S) plasma as in panels A-D, in absence or presence of the FATP1 inhibitor, FATP1in2, and assessed for IFN-γ expression by ELISA. Significance was calculated using Wilcoxon matched-pairs signed rank test for panels A-H (circles), I-J,N-O,Q-R; and paired t test for panels K,S; ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .0001. UMAP, Uniform Manifold Approximation and Projection.

Journal: Blood Advances

Article Title: Uptake of long-chain fatty acids from the bone marrow suppresses CD8 + T-cell metabolism and function in multiple myeloma

doi: 10.1182/bloodadvances.2023009890

Figure Lengend Snippet: CD8 + T-cell function is impaired by BM long-chain fatty acid uptake via FATP1. (A-D) BM mononuclear cells from controls (n = 6) or patients with MM who had relapsed (n = 6) were cultured in autologous PB or BM plasma as indicated for 72 hours with stimulation via CD3/CD28. (A) CD8 + T-cell mitochondrial mass was assessed by flow cytometry using MVG and is shown for each group (left) and both groups combined (right). (B) At 72 hours, T cells were activated with PMA/ionomycin and expression of IFN-γ was assessed within CD8 + T cells by flow cytometry. (C-D) IFN-γ and TNF-α in cell culture supernatants was measured by ELISA. (E-H) BM mononuclear cells were cultured and assessed as in panels A-D but in either complete or lipid-depleted BM autologous plasma as indicated. (I-K) BM mononuclear cells from patients with MM at diagnosis (n = 6) were cultured in autologous PB (I,K) or BM (J-K) plasma as in panels A-D, in absence or presence of ferrostatin and assessed for IFN-γ expression by ELISA. (L) Proportion of CD8 + T cells expressing CD36 within indicated BM mononuclear cell samples from controls (n = 10), individuals with MGUS (n = 8), asymptomatic MM (asymptomatic; n = 9), or MM at diagnosis (diagnosis; n = 7) were assessed by flow cytometry. (M) Expression of IFN-γ, TNF-α, and IL-2 by CD36 + or CD36 − CD8 + T cells within indicated BM mononuclear cell samples as in panel L. (N-O) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous BM plasma as in panel A-D, in absence or presence of the CD36 inhibitor, SSO, and assessed for IFN-γ expression within CD8 + T cells by flow cytometry (N) or by ELISA (O). (P) single-cell RNA-sequencing UMAP projections of BM mononuclear cells from 2 patients with newly diagnosed MM, with cell type annotation (top left) and transcript abundance of indicated genes overlaid. (Q-S) BM mononuclear cells from patients with MM at diagnosis (n = 7) were cultured in autologous PB (Q,S) or BM (R-S) plasma as in panels A-D, in absence or presence of the FATP1 inhibitor, FATP1in2, and assessed for IFN-γ expression by ELISA. Significance was calculated using Wilcoxon matched-pairs signed rank test for panels A-H (circles), I-J,N-O,Q-R; and paired t test for panels K,S; ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .0001. UMAP, Uniform Manifold Approximation and Projection.

Article Snippet: Where indicated, BM plasma was stripped of lipids using CleanAscite (Caltag, catalog #X2555-10), or additions were made: SSO (CD36 agonist, 100 μM; Cambridge Biosciences catalog #11211), ferrostatin (inhibitor of lipid peroxidation, 1 μM; Selleckchem, catalog #S7243), fatty acid transport protein 1 (FATP1)-IN-2 (an FATP1 inhibitor, 1 μM; Medchem express, catalog #HY-141700), or ursodeoxycholic acid (an FATP5 inhibitor, 10 μM; Sigma, catalog #PHR1579).

Techniques: Cell Function Assay, Cell Culture, Clinical Proteomics, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Single Cell, RNA Sequencing